Online tool for designing gRNA for Base editor-mediated Inactivation of Genes (gBIG)

gBIG designs guide RNAs for base editing-mediated inactivation of given genes in a given sequenced genome.

PAM Type

CRISPR/Cas variant

Base Editor Type

1 Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. 2016. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science 353:aaf8729.

2 Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. 2016. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533:420–424.

Reference Genome

Upload target gene

Fasta format. Sequence of expressed region is required for eukaryotic genes. Targets are limited to 30,000 bases of raw DNA sequence.