TIB gBIG

Email:

Online tool for designing gRNA for Base editor-mediated Inactivation of Genes (gBIG)

gBIG designs guide RNAs for base editing-mediated inactivation of given genes in a given sequenced genome.

User Guide

PAM Type

CRISPR/Cas variant

SpCas9 from Streptococcus pyogenes: 5'-NGG-3'

SpCas9 from Streptococcus pyogenes: 5'-NAG-3'

VRER SpCas9 from Streptococcus pyogenes: 5'-NGCG-3'

VQR SpCas9 from Streptococcus pyogenes: 5'-NGA-3'

xCas9 3.7 (TLIKDIV SpCas9) from Streptococcus pyogenes: 5'-NGT-3'

SpCas9-NG (VRVRFRR SpCas9) from Streptococcus pyogenes: 5'-NG-3'

Base Editor Type

Target-AID¹

BE²

¹ Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. 2016. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science 353:aaf8729.

² Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. 2016. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533:420–424.

Reference Genome

Genomes

Escherichia coli str. K-12 substr. MG1655

Corynebacterium glutamicum ATCC 13032

Bacillus subtilis 168

Saccharomyces cerevisiae S288C

Shewanella Oneidensis MR-1

Myceliophthora thermophila

Aeromonas hydrophila subsp. hydrophila ATCC 7966

Corynebacterium glutamicum ATCC 13869

Fos11

Geobacter sulfurreducens PCA

Komagataeibacter xylinus CGMCC 2955

Komagataella phaffii GS115

YB1

Bacillus methanolicus MGA3

Upload target gene

Fasta format. Sequence of expressed region is required for eukaryotic genes. Targets are limited to 30,000 bases of raw DNA sequence.

Upload inactivated target gene:

Email address: